Microbial Biodiversity of the Built Environment.
Determine the highly dynamic microbiome and it's impact.
The new frontier for building & occupancy health
Each building has its own specific yet highly dynamic microbiome whose composition (type and concentration of microorganisms) is affected by multiple parameters including the presence of people and animals, types and quantity of plants, ventilation efficiency, surrounding climate and biodiversity, etc.
An indoor air microbiome is a collection of microorganisms that can be found in the air of a building.
Modern metrics for new insights
For the analysis of the microbial biodiversity in the indoor air, samples are collected with the air sampler. The collected air samples are further processed according to the scheme below
1. Sample collection
Strategic and representative locations are determined throughout the building
2. DNA & RNA extraction
Nucleic acid extraction and purification from air samples
3. Next-generation sequencing
Determine the order of nucleotides of targeted regions for DNA and RNA
4. Bio-informatics analysis
Computation and analysis interpretation of captured biological data
5. Taxonomic assignment of MO
Aligning the sequence reads to a set of reference sequences, resolving any ambiguities
6. Patterns and correlation analysis
Identifying the intersection of our scoped datasets
For more information about the development of Indoor Microbial Diversity program contact sylwia.jezierska@takeair.world
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